Direct evidence of a role for Nox2 in superoxide production, reduced nitric oxide bioavailability, and early atherosclerotic plaque formation in ApoE -/- mice

CP Judkins; H Diep; BRS Broughton; AE Mast; EU Hooker; AA Miller; S Selemidis; GJ Dusting; CG Sobey; GR Drummond

Journal article

Direct evidence of a role for Nox2 in superoxide production, reduced nitric oxide bioavailability, and early atherosclerotic plaque formation in ApoE -/- mice

CP Judkins, H Diep, BRS Broughton, AE Mast, EU Hooker, AA Miller, S Selemidis, GJ Dusting, CG Sobey, GR Drummond

American Journal of Physiology Heart and Circulatory Physiology | Published : 2010

DOI: 10.1152/ajpheart.00799.2009

Abstract

The Nox family NADPH oxidases are reactive oxygen species (ROS)-generating enzymes that are strongly implicated in atherogenesis. However, no studies have examined which Nox isoform(s) are involved. Here we investigated the role of the Nox2-containing NADPH oxidase in atherogenesis in apolipoprotein E-null (ApoE-/-) mice. Wild-type (C57Bl6/J), ApoE-/-, and Nox2-/y/ApoE-/- mice were maintained on a high-fat (21%) diet from 5 wk of age until they were 12 or 19 wk old. Mice were euthanized and their aortas removed for measurement of Nox2 expression (Western blot analysis and immunohistochemistry), ROS production (L012-enhanced chemiluminescence), nitric oxide (NO) bioavailability (contractions ..

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University of Melbourne Researchers

Gregory Dusting Author

Related Projects (2)

Research Fellowship

Targetting the NAPDHoxidase source of reactive oxygen species in vascular disease

In Australia, coronary heart disease (CHD) leading to heart attacks or strokes is the largest cause of death, claiming a staggering 28,000 l..

Grants

Awarded by National Health and Medical Research Council of Australia

Awarded by National Heart Foundation of Australia

Funding Acknowledgements

This work was supported by National Health and Medical Research Council of Australia Project Grants 300013 and 384163 and Fellowships 465109 (to G. R. Drummond), 350327 (to C.G. Sobey), 400303 (to G.J. Dusting), and 232324 (to S. Selemidis); National Heart Foundation of Australia Grants PF06M2781 (to C.P. Judkins) and PF07M3289 (to B.R.S. Broughton); and a High Blood Pressure Research Council of Australia fellowship (to A.A. Miller).